Regulatory
Part:BBa_K305007:Design
Designed by: Joel Kuiper, Arend Jan Suk Group: iGEM10_Groningen (2010-10-18)
srfA promoter, short variant
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence was retrieved from genomic Bacillus subtilis DNA using PCR with the following primers:
Srfa-short-for: ATCAGAATTCGCGGCCGCTTCTAGAGGATTGAACGCAGCAGTTTG
Srfa-short-rev: ACTACTGCAGCGGCCGCTACTAGTACCCACCCTAATAAGAAACC
Source
[http://dbtbs.hgc.jp/COG/prom/srfAA-srfAB-comS-srfAC-srfAD.html dbts]
[http://subtiwiki.uni-goettingen.de/wiki/index.php/SrfAA SubtiWiki SrfAA]