Regulatory

Part:BBa_K305007:Design

Designed by: Joel Kuiper, Arend Jan Suk   Group: iGEM10_Groningen   (2010-10-18)

srfA promoter, short variant


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was retrieved from genomic Bacillus subtilis DNA using PCR with the following primers:

Srfa-short-for: ATCAGAATTCGCGGCCGCTTCTAGAGGATTGAACGCAGCAGTTTG

Srfa-short-rev: ACTACTGCAGCGGCCGCTACTAGTACCCACCCTAATAAGAAACC


Source

[http://dbtbs.hgc.jp/COG/prom/srfAA-srfAB-comS-srfAC-srfAD.html dbts]

[http://subtiwiki.uni-goettingen.de/wiki/index.php/SrfAA SubtiWiki SrfAA]

References